What does dextran label?
The 500,000 MW and 2,000,000 MW dextrans are labeled with fluorescein, tetramethylrhodamine or Texas Red dyes or with biotin, and all contain aldehyde-fixable lysine groups.
Is dextran fluorescent?
Fluorescent amino dextrans, also known as “fixable”, contain amino groups which can be reacted with crosslinking reagents such as glutaraldehyde. Fluorescent dextrans do not have any residual free amine groups. Dextrans with other fluorescent dyes available as custom products.
What is FITC dextran?
FITC-Dextran are polysaccharides comprised of varying lengths of branched glucose molecules and can be used to determine solute, ion and protein permeability of the blood-brain barrier (BBB) based on the size of the dextran used. For research use only.
How do you precipitate dextran?
DEXTRANS AND DEXTRAN DERIVATIVES WHICH ARE READILY SOLUBLE IN COLD WATER ARE PRODUCED BY ADDING A SIRUPY ALCOHOL- OR KETONE-WATER MIXTURE OF DEXTRAN OR A DEXTRAN DERIVATIVE TO A HIGHLY CONCENTRATED ALCOHOL OR KETONE TO PRODUCE A GRANULAR PRECIPITATE WHICH IS WASHED WITH THE RESPECTIVE CONCENTRATED ALCOHOL OR KETONE.
What is Texas red dextran?
Description: Texas red labeled Dextrans are one of Nanocs fluorescent dextran conjugates with far red fluorescence. Dextrans labeled with Texas red dye have excitation/emission wavelength at 594 nm/616 nm.
Is dextran cell permeable?
Abstract: Dextran does not penetrate the living cells. However when the plasma membrane becomes permeable, it is concentrated in the cells. This is why we used fluorescein isothiocyanate-conjugated dextran (FITC-Dextran, MW 4000) to identify apoptotic and necrotic cells by flow cytometry.
How do you dissolve dextran in water?
Dextran fractions are freely soluble in water. To hasten dissolution of dextran powders, the powder should be added portion wise to warm (hot) water with vigorous stirring to avoid lump formation.
Is dextran water soluble?
Dextran is a natural branched bacterial polysaccharide produced by Leuconostoc mesenteroides that consists of α-1,6-d-glucopyranose with α-1,2, α-1,3, or α-1,4 linked side chains (Jiang et al., 2004). It is soluble in water and organic solvents.
What is dextran made of?
Dextran is a complex branched glucan (polysaccharide made of many glucose molecules). Dextran is synthetized from sucrose by certain lactic-acid bacteria, the best known are Leuconostoc bacteroides and Streptococcus mutans.
What laser does Texas Red use?
Invitrogen Texas Red dye is a bright red-fluorescent dye with excitation ideally suited to the 561 or 594 nm laser lines. It is commonly conjugated to antibodies and proteins for cellular imaging applications.
How does fluorescein work in the eye?
A piece of blotting paper containing the dye is touched to the surface of your eye. You are asked to blink. Blinking spreads the dye and coats the tear film covering the surface of the cornea. The tear film contains water, oil, and mucus to protect and lubricate the eye.