What is pUC19 vector?

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pUC19 is a commonly used cloning vector that conveys the Amp resistance. The molecule is a small double-stranded circle, 2686 base pairs in length, and has a high copy number.

What is pUC18 19?

pUC18 / pUC19 from E. coli are commonly used small, high copy number, E. coli plasmids, 2686 bp in length. pUC19 is identical to pUC18 except that they contain multiple cloning sites (MCS) arranged in opposite orientations.

What features make pUC18 and 19 particularly useful?

The pUC18 and pUC19 plasmids enable successful cloning of large DNA fragments (larger than those cloned with a M13 mp18 RF Phage Vector).

Which antibiotic resistance gene is present in the pUC18 vector?

Ampicillin
The artificial plasmid pUC18 has been genetically engineered to include (1) a gene for antibiotic resistance to Ampicillin (amp R), and (2) a gene (and its promoter) for the enzyme beta-galactosidase (lacZ).

What is the primary difference between pBR322 and pUC series of vectors?

The key difference between pBR322 and pUC19 is that pBR322 is a plasmid vector that is 4361 base pairs in length, while pUC19 is a plasmid vector that is 2686 base pairs in length.

What is the function of rop in pBR322?

ROP codes for a protein that is responsible for restricting the plasmid copy number. It is also called a repressor for primers. It is involved in the replication of the plasmid pBR322.

What are the advantages of pUC vectors over pBR322?

The pUC vectors offer following major advantages over pBR322 vectors: (a) High copy number of 500-600 copies per cell. (b) Easy and single step selection. (c) The unique restriction sites used for cloning are clustered within the MCS.

How was pBR322 constructed?

The plasmid was constructed with genetic material from 3 main sources – the tetracycline resistance gene of pSC101, the ampicillin resistance gene of RSF 2124, and the replication elements of pMB1, a close relative of the ColE1 plasmid.

What is the role of Rop protein in plasmid?

Rop (also known as repressor of primer, or as RNA one modulator (ROM)) is a small dimeric protein responsible for keeping the copy number of ColE1 family and related bacterial plasmids low in E.

What is the role of rop in the plasmid?

Repressor of primer (Rop) is a small dimeric protein that participates in the mechanism that controls the copy number of plasmid of the ColE1 family by increasing the affinity between two complementary RNAs.

What are pUC vectors?

pUC19 is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. The designation “pUC” is derived from the classical “p” prefix (denoting “plasmid”) and the abbreviation for the University of California, where early work on the plasmid series had been conducted.

Is pBR322 artificially constructed cloning vector?

pBR322 was the first artificial cloning vector to be constructed.

What is pUC18 plasmid?

pUC18 is a commonly used plasmid cloning vector in E.coli. The molecule is a double-stranded circular DNA (2686 base pairs in length). Due to a small size pUC18 enables successful cloning of large DNA fragments. The pUC18 plasmid confers ampicillin resistance and complement defects in β-galactosidase in appropriate host strains.

What is pUC18 used for?

What is the MCs of pUC19?

Standard E. coli vector with a multiple cloning site (MCS) for DNA cloning. The MCS is reversed in pUC19. To see this sequence with restriction sites, features, and translations, please download SnapGene or the free SnapGene Viewer.

Which plasmids are suitable for dideoxy DNA sequencing using M13 primer?

The pUC18 and pUC19 plasmids are suitable for dideoxy DNA sequencing using M13 primers. To sequence pUC18 and pUC19 plasmids containing large DNA insertions, utilize the Deletion Kit for Kilo-Sequencing (Cat. # 6030). This DNA is a plasmid vector suitable for DNA sequencing by dideoxy method.